| 1 | Po Lazarus | James Carter And The Prisoners | 04:32 |
| 2 | Big Rock Candy Mountain | Harry McClintock | 02:18 |
| 3 | You Are My Sunshine | Norman Blake | 04:28 |
 | 4 | Down to the River to Pray | Alison Krauss | 02:56 |
| 5 | Man of Constant Sorrow | The Soggy Bottom Boys | 03:10 |
| 6 | Hard Time Killin' Floor Blues | Chris Thomas King | 02:43 |
| 7 | Man of Constant Sorrow | Norman Blake | 04:30 |
| 8 | Keep on the Sunny Side | The Whites | 03:35 |
| 9 | I'll Fly Away | Alison Krauss | 03:58 |
| 10 | Didn't Leave Nobody But the Baby | Alison Krauss | 01:58 |
| 11 | In the Highways | Hannah Peasall | 01:36 |
| 12 | I Am Weary, Let Me Rest | The Cox Family | 03:14 |
| 13 | Man of Constant Sorrow | John Hartford | 02:34 |
| 14 | Oh Death | Ralph Stanley | 03:20 |
| 15 | In the Jailhouse Now | The Soggy Bottom Boys | 03:36 |
| 16 | Man of Constant Sorrow [Band Version] | The Soggy Bottom Boys | 04:17 |
| 17 | Indian War Whoop | John Hartford | 01:31 |
| 18 | Lonesome Valley | The Fairfield Four | 04:07 |
| 19 | Angel Band | The Stanley Brothers | 02:18 |