| 1 | On the Road Again | Memphis Jug Band | 02:52 |
| 2 | Dark Night Blues | Blind Willie McTell | 02:55 |
| 3 | Minglewood Blues | Cannon's Jug Stompers | 03:42 |
| 4 | Hard Time Killin' Floor Blues | Skip James | 02:50 |
| 5 | I'm Gonna Cross the River of Jordan - Some O' These Days | Jaybird Coleman | 03:08 |
| 6 | High Water Everywhere | Charley Patton | 03:08 |
| 7 | I Got Mine | Frank Stokes | 03:07 |
| 8 | Sugar Baby | "Docks" Boggs | 03:00 |
| 9 | Big Bend Gal | Shelor Family | 02:49 |
| 10 | The Peddler and His Wife | Hayes Shepherd | 03:22 |
| 11 | Little Rabbit | Crockett's Kentucky Mountaineers | 03:01 |
| 12 | All Night Long Blues | Burnett & Rutherford | 03:15 |
| 13 | Mineola Rag | East Texas Serenaders | 02:44 |
| 14 | Greenback Dollar | Weems String Band | 03:09 |
| 15 | Kater Street Rag | Bennie Moten's Kansas City Orchestra | 02:34 |
| 16 | Sobbin' Blues | "King" Oliver's Creole Jazz Band | 03:19 |
| 17 | Mojo Strut | Parham-Pickett Apollo Syncopators | 02:49 |
| 18 | Somebody Stole My Gal | Frankie Franko & His Louisianians | 02:30 |
| 19 | Wild Cat Blues | Clarence Williams' Blue Five | 03:02 |
| 20 | Kansas City Stomps | "Jelly Roll" Morton's Red Hot Peppers | 02:56 |
| 21 | King Joe [glitched] | Jimmy Noone | 03:03 |